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KMID : 1134820190480010032
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 1 p.32 ~ p.39
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Antioxidant Activities of Peucedanum japonicum Thunberg Root Extracts
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Lim Hyun-Hwa
Kim In-Yong
Jeong Yoon-Hwa
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Abstract
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The antioxidant activities of Peucedanum japonicum Thunberg root extracts were investigated in oxidatively stressed HepG2 cells. P. japonicum Thunberg root was extracted with distilled water (PJRDE) and 95% ethanol (PJREE); their antioxidant activities were analyzed in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, the Ferric reducing antioxidant power (FRAP) assay, and the oxygen radical absorbance capacity (ORAC) assay. The extracts were evaluated for cellular anti-oxidation and protection using cell viability, intracellular reactive oxygen species (ROS) scavenging activity, and antioxidant enzyme activities. PJREE contained high amounts of bio-active compounds such as total phenol (5.64 mg gallic acid equivalent/g) and flavonoid (664.2 μg quercetin equivalent/g) and high antioxidant activities in the DPPH radical scavenging assay (95.5% at 1,000 μg/mL), the ABTS radical scavenging assay (87.2% at 2,000 μg/mL), FRAP (39.8 μmol FeSO4/g), and ORAC (133.4 μmol TE/g). The extracts also enhanced antioxidant enzyme activity such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the oxidatively stressed HepG2 cells. The activities of SOD, CAT, GPx, and GR were measured at 85.8%, 68.7%, 90.8%, and 105.4%, respectively, with PJREE (1,000 μg/mL). These results suggest that the extract (PJREE) strongly induces antioxidant activity of the enzymes including SOD, CAT, GPx, and GR and is composed of effective antioxidant compounds against H2O2-stressed HepG2 cells.
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KEYWORD
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Peucedanum japonicum Thunberg, oxidative stress, antioxidant activity, HepG2 cell, superoxide
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